Clinical Pathology and Microbiology Unit

 Clinical Activities

The Clinical Pathology and Microbiology Laboratory Unit deals with the diagnosis of infectious disease serology testing such as microbiology, mycology and virology and immunological serology, cellular immunology and allergology diseases.

A large part of the Laboratory’s activities is devoted to collaborating with the medical departments for elaborating publically funded and targeted research lines as well as projects with the National Institute of Health (Istituto Superiore di Sanità).

 Collaborators

 
Lab technicians
Sabrina Volpi
Serena Argentino
E-mail   serena.argentino@ifo.gov.it

Dr.ssa Laura Cilli
E-mail   laura.cilli@ifo.gov.it

Chemical
Dr.ssa Francesca Stivali 
E-mail   francesca.stivali@ifo.gov.it  

Secretariat
Sig.ra Luisa Scherillo

Tel. 06 52665069   E-mail luisa.scherillo@ifo.gov.it 

 Medical Visits/Exams

 This Unit carries out diagnostic activities in the following areas:

 
Allergology:
Total serum IgE, Specific IgE to inhalant allergens , common food allergy, hymenoptera and antibiotics, Eosinophil cationic protein (ECP) test.

Immunology:

Serological and immunoistochemistry examinations for the diagnosis of autoimmune systemic and organ-specific diseases (inflammation of the connective tissue, celiac disease, bullous diseases, neuropathies, hepatitis, vasculitis); molecular analyses to determine and detect HLA-DR2 and DR4/DQ8 polimorphism with the risk of celiac disease; molecular analysis for the diagnosis of lymphoproliferative cutaneous pathologies (molecular rearrangement of the T-cell Receptor, rearrangement of the heavy chains of immunoglobulines); cytofluorimetric analysis for the immunophenotyping of lymphocytes.

Infectious diseases:
Serological research for infections resulting from exposure to herpetic viruses (CMV, EBV, HHV-8, HSV1 e HSV2), hepatic (HAV, HBV, HCV), virus HIV-1, for serodiagnosis of syphilis, lyme disease, toxoplasmosis and rubella; molecular analyses to define plasmatic viremia from HIV, HBV, HCV, CMV (quantitative analysis) and HHV-6, EBV, VHZ (qualitative analysis).

Microbiology
:
Microscopic and culture exams of germs and fungi, antibiograms and antimicograms. Molecular analyses to define the genome of M. Tuberculosis, Treponema pallidum, Chlamydia trachomatis, HSV1, HSV2, HPV (genotyping) in various biological samples ( bronchial lavage, sputum, urine, lesional swabs , endocervical or endouretral).

The Microbiology Unit carries out (open from Mondays to Fridays from 8:30 to 13:00) withdrawals (without appointment) as well as microscopic tests and cultures for fungi.

 Where/How to gain access to the Division

Qui rimandiamo agli orari generali di prelievo ed accettazione, non ci sono esami su prenotazione.

 

 Research Projects

Publicly funded research activities has concerned the following areas:


Inflammatory pathologies
:

a) Atopic dermatitis: specific serum IgE for milk proteins, ECP, cytokines and cutaneous homing receptors in pediatric patients with atopic dermatitis.
b) Psoriasis: doses of cytokines and cutaneous homing receptors, evaluation of modified serum levels of biological mediators (interleukins) in patients with arthropatic psoriasis being treated with biological immune response modifiers; research the presence of genoma virus of HPV-5 in healthy skin and skin lesions.

Infectious diseases:
a) HHV-8 infection: standardization of the IFA method for the specific antibody dose and retrospective study regarding the incidence of HHV-8 infection in subjects connected to STDs Center; study of cellular immunity in patients with Kaposi disease.
b) HIV infection: MST infections in HIV-positive patients.
c) HPV infection: studies regarding viral genome and genotyping.
For diagnosing infectious diseases the following exams are carried out: immunofluorescence, immunoenzimatic tests, hemoagglutination, WBA, RIBA, nested PCR, RT-PCR.


Oncological diseases:
a) For the detection of tyrosinase mRNA in sentinel nodes of melanoma patients a molecular analysis and an immunohistochemical analysis are performed.
b) For the detection of TCR gamma chain gene rearrangement in TCR in lymphoproliferative skin lesions (LPP, MF).